Analysis of the status of excess heart age and its risk factors among residents aged 35 to 64 years in China / 中华预防医学杂志

Objective: To analyze the status of excess heart age and its risk factors among Chinese residents aged 35 to 64 years. Methods: The study subjects were Chinese residents aged 35 to 64 years who completed the heart age assessment by WeChat official account "Heart Strengthening Action" through the internet from January 2018 to April 2021. Information such as age, gender, body mass index (BMI), blood pressure, total cholesterol (TC), smoking history, and diabetes history was collected. The heart age and excess heart age were calculated according to the characteristics of individual cardiovascular risk factors and the heart aging was defined as excess heart age≥5 years and 10 years respectively. The heart age and standardization rate were calculated respectively based on the population standardization of the 7th census in 2021.CA trend test was used to analyze the changing trend of excess heart age rate and population attributable risk (PAR) was used to calculate the contribution of risk factors. Results: The mean age of 429 047 subjects was (49.25±8.66) years. The male accounted for 51.17% (219 558/429 047) and the excess heart age was 7.00 (0.00, 11.00) years. The excess heart age rate defined by excess heart age≥5 years and ≥10 years was 57.02% (the standardized rate was 56.83%) and 38.02% (the standardized rate was 37.88%) respectively. With the increase of the age and number of risk factors, the excess heart age rate of the two definitions showed an upward trend according to the result of the trend test analysis (P<0.001). The top two risk factors of the PAR for excess heart age were overweight or obese and smoking. Among them, the male was smoking and overweight or obese, while the female was overweight or obese and having hypercholesterolemia. Conclusion: The excess heart age rate is high in Chinese residents aged 35 to 64 years and the contribution of overweight or obese, smoking and having hypercholesterolemia ranks high.

Analysis of the status of excess heart age and its risk factors among residents aged 35 to 64 years in China / 中华预防医学杂志

Objective: To analyze the status of excess heart age and its risk factors among Chinese residents aged 35 to 64 years. Methods: The study subjects were Chinese residents aged 35 to 64 years who completed the heart age assessment by WeChat official account"Heart Strengthening Action"through the internet from January 2018 to April 2021. Information such as age, gender, body mass index (BMI), blood pressure, total cholesterol (TC), smoking history, and diabetes history was collected. The heart age and excess heart age were calculated according to the characteristics of individual cardiovascular risk factors and the heart aging was defined as excess heart age≥5 years and 10 years respectively. The heart age and standardization rate were calculated respectively based on the population standardization of the 7th census in 2021.CA trend test was used to analyze the changing trend of excess heart age rate and population attributable risk (PAR) was used to calculate the contribution of risk factors. Results: The mean age of 429 047 subjects was 49.25±8.66 years. The male accounted for 51.17% (219 558/429 047) and the excess heart age was 7.00 (0.00, 11.00) years. The excess heart age rate defined by excess heart age≥5 years and≥10 years was 57.02% (the standardized rate was 56.83%) and 38.02% (the standardized rate was 37.88%) respectively. With the increase of the age and number of risk factors, the excess heart age rate of the two definitions showed an upward trend according to the result of the trend test analysis (P<0.001). The top two risk factors of the PAR for excess heart age were overweight or obese and smoking. Among them, the male was smoking and overweight or obese, while the female was overweight or obese and having hypercholesterolemia. Conclusion: The excess heart age rate is high in Chinese residents aged 35 to 64 years and the contribution of overweight or obese, smoking and having hypercholesterolemia ranks high.

Reliability and validity of the solitude behavior scale for short version / 中华行为医学与脑科学杂志

Objective:To develop a suitable solitude behavior scale for short version(SBS-S).Methods:A total of 2 484 valid data were collected from college students in Tianjin city and Shandong province.Based on the exploratory structural equation model(ESEM) and generalized partial credit model(GPCM) of item response theory, the SBS-S was developed.A total of 100 college students in Guizhou province were tested twice at intervals of two months to calculate the test-retest reliability of the SBS-S.R language Mplus 8.0 and SPSS 24.0 software were used for the data analysis.Results:According to the results of ESEM and GPCM model, 18 items were deleted. After deleting the above questions, 16 items (four items under each dimension) and four dimensions(positive solitude, eccentricity, social avoidance and loneliness) were retained. The coefficient of Cronbach's α was above 0.75. The results of confirmatory factor analysis showed that the structure model of the SBS-S was fitting well, and the index was ( χ2/ df=4.74, TLI=0.92, CFI= 0.95, SRMR =0.05, RMSEA=0.06). According to the results of SPSS, the test-retest reliability was above 0.60.Big-five persondlity, social support and self-esteem were regarded as criterion related validity. The results showed that positive solitude had positive correlations with extraversion, conscientiousness, agreeableness, openness and self-esteem( r=0.07, 0.25, 0.15, 0.26, 0.09, all P<0.05). Eccentricity and social avoidance had negative correlations with extraversion, conscientiousness, agreeableness, social support and self-esteem( r=-0.25, -0.22, -0.45, -0.16, -0.36, all P<0.05; r=-0.26, -0.09, -0.06, -0.18, -0.36, all P<0.05), and had positive correlations with neuroticism( r=0.25, 0.32, both P<0.05). Loneliness had negative relation with extraversion, conscientiousness, agreeableness and self-esteem( r=-0.08, -0.11, -0.08, -0.33, all P<0.05), and had positive correlations with neuroticism( r=0.33, P<0.05). Conclusion:The SBS-S has good psychometric characteristics and can be used to evaluate the solitude behavior of individuals.

Effects of exosomes from human adipose-derived mesenchymal stem cells on inflammatory response of mouse RAW264.7 cells and wound healing of full-thickness skin defects in mice / 中华烧伤杂志

Objective: To investigate the effects of exosomes from human adipose-derived mesenchymal stem cells (ADSCs) on inflammatory response of mouse RAW264.7 cells and wound healing of full-thickness skin defects in mice. Methods: The experimental research methods were adopted. The discarded adipose tissue was collected from 3 female patients (aged 10-25 years) who underwent abdominal surgery in the First Affiliated Hospital of Air Force Medical University. ADSCs were extracted from the adipose tissue by collagenase Ⅰ digestion and identified with flow cytometry. Exosomes were extracted from the human ADSCs by differential ultracentrifugation, the morphology of the exosomes was observed by transmission electron microscopy, the particle diameter of the exosomes was detected by nanoparticle tracking analyzer, and the protein expressions of CD9, CD63, tumor susceptibility gene 101 (TSG101), and β-actin were detected by Western blotting. The human ADSCs exosomes (ADSCs-Exos) and RAW264.7 cells were co-cultured for 12 h, and the uptake of RAW264.7 cells for human ADSCs-Exos was observed. The RAW264.7 cells were divided into phosphate buffer solution (PBS) group stimulated with PBS for suitable time, endotoxin/lipopolysaccharide (LPS) stimulation 2 h group, LPS stimulation 4 h group, LPS stimulation 6 h group, LPS stimulation 12 h group, and LPS stimulation 24 h group stimulated with LPS for corresponding time, with 3 wells in each group, and the mRNA expressions of interleukin 1β (IL-1β), tumor necrosis factor α (TNF-α), IL-6, and IL-10 were detected by real-time fluorescence quantitative reverse transcription polymerase chain reaction (RT-PCR) method. The RAW264.7 cells were divided into PBS group, LPS alone group, and LPS+ADSCs-Exos group, with 3 wells in each group, which were dealt correspondingly for the time screened out in the previous experiment, the mRNA expressions of IL-1β, TNF-α, IL-6, IL-10, trasforming growth factor β (TGF-β,) and vascular endothelial growth factor (VEGF) were detected by real time fluorescence quantitative RT-PCR method, and the protein expressions of inducible nitric oxide synthase (iNOS) and arginase 1 (Arg1) were detected by Western blotting. Twenty-four 8-week-old male BALB/c mice were divided into PBS group and ADSCs-Exos group according to the random number table, with 12 mice in each group, and a full-thickness skin defect wound with area of 1 cm×1 cm was inflicted on the back of each mouse. Immediately after injury, the wounds of mice in the two groups were dealt correspondingly. On post injury day (PID) 1, the concentration of IL-1β and TNF-α in serum were detected by enzyme-linked immunosorbent assay, and the mRNA expressions of IL-1β, TNF-α, and IL-6 were detected by real time fluorescence quantitative RT-PCR method. On PID 3, 6, 9, 12, and 15, the wound healing was observed and the wound non-healing rate was calculated. On PID 15, the defect length of skin accessory and collagen volume fraction (CVF) were detected by hematoxylin eosin staining and Masson staining, respectively, the CD31 expression and neovascularization were detected by immunohistochemistry, and the ratio of Ki67 positive cells, the ratio of iNOS and Arg1 double positive cells, and the ratio of iNOS positive cells to Arg1 positive cells and their fluorescence intensities were detected by immunofluorescence method. The number of samples in animal experiments was 6. Data were statistically analyzed with analysis of variance for repeated measurement, one-way analysis of variance, and independent sample t test. Results: At 12 h of culture, the cells exhibited a typical spindle shape, which were verified as ADSCs with flow cytometry. The exosomes with a vesicular structure and particle diameters of 29-178 nm, were positively expressed CD9, CD63, and TSG101 and negatively expressed β-actin. After 12 h of co-culture, the human ADSCs-Exos were endocytosed into the cytoplasm by RAW264.7 cells. The mRNA expressions of IL-1β, TNF-α, IL-6, and IL-10 of RAW264.7 cells in LPS stimulation 2 h group, LPS stimulation 4 h group, LPS stimulation 6 h group, LPS stimulation 12 h group, and LPS stimulation 24 h group were significantly higher than those in PBS group (with t) values of 39.10, 14.55, 28.80, 4.74, 48.80, 22.97, 13.25, 36.34, 23.12, 18.71, 29.19, 41.08, 11.68, 18.06, 8.54, 43.45, 62.31, 22.52, 21.51, and 37.13, respectively, P<0.01). The stimulation 12 h with significant expressions of all the inflammatory factors was selected as the time point in the following experiment. After stimulation of 12 h, the mRNA expressions of IL-1β, TNF-α, IL-6, and IL-10 of RAW264.7 cells in LPS alone group were significantly higher than those in PBS group (with t values of 44.20, 51.26, 14.71, and 8.54, respectively, P<0.01); the mRNA expressions of IL-1β, TNF-α, and IL-6 of RAW264.7 cells in LPS+ADSCs-Exos group were significantly lower than those in LPS alone group (with t values of 22.89, 25.51, and 8.03, respectively, P<0.01), while the mRNA expressions of IL-10, TGF-β, and VEGF were significantly higher than those in LPS alone group (with t values of 9.89, 13.12, and 7.14, respectively, P<0.01). After stimulation of 12 h, the protein expression of iNOS of RAW264.7 cells in LPS alone group was significantly higher than that in PBS group and LPS+ADSCs-Exos group, respectively (with t values of 11.20 and 5.06, respectively, P<0.05 or P<0.01), and the protein expression of Arg1 was significantly lower than that in LPS+ADSCs-Exos group (t=15.01, P<0.01). On PID 1, the serum concentrations of IL-1β and TNF-α and the mRNA expressions of IL-1β, TNF-α, and IL-6 in wound tissue of mice in ADSCs-Exos group were significantly those in lower than PBS group (with t values of 15.44, 12.24, 9.24, 7.12, and 10.62, respectively, P<0.01). On PID 3, 6, 9, 12, and 15 d, the wound non-healing rates of mice in ADSCs-Exos group were (73.2±4.1)%, (53.8±3.8)%, (42.1±5.1)%, (24.1±2.8)%, and 0, which were significantly lower than (82.5±3.8)%, (71.2±4.6)%, (52.9±4.1)%, (41.5±3.6)%, and (14.8±2.5)% in PBS group, respectively (with t values of 4.77, 8.93, 5.54, 7.63, and 7.59, respectively, P<0.01). On PID 15, the defect length of skin accessory in wounds of mice in PBS group was significantly longer than that in ADSCs-Exos group (t=9.50, P<0.01), and the CVF was significantly lower than that in ADSCs-Exos group (t=9.15, P<0.01). On PID 15, the CD31 expression and the number of new blood vessels (t=12.99, P<0.01), in wound tissue of mice in ADSCs-Exos group were significantly more than those in PBS group, and the ratio of Ki67 positive cells was significantly higher than that in PBS group (t=7.52, P<0.01). On PID 15, the ratio of iNOS and Arg1 double positive cells in wound tissue of mice in PBS group was (12.33±1.97)%, which was significantly higher than (1.78±0.29)% in ADSCs-Exos group (t=13.04, P<0.01), the ratio of iNOS positive cells and the fluorescence intensity of iNOS were obviously higher than those of ADSCs-Exos group, and the ratio of Arg1 positive cells and the fluorescence intensity of Arg1 were obviously lower than those of ADSCs-Exos group. Conclusions: The human ADSCs-Exos can alleviate inflammatory response of mouse RAW264.7 cells, decrease macrophage infiltration and secretion of the pro-inflammatory cytokines, increase the secretion of anti-inflammatory cytokines to promote neovascularization and cell proliferation in full-thickness skin defect wounds of mice, hence accelerating wound healing.

Analysis of the correlative characteristics of national occupational health standards based on social network analytic method / 中国职业医学

OBJECTIVE: To explore the correlative characteristics of the National Occupational Health Standards by social network analytic method. METHODS: The national occupational health standards were searched and collected through the National Health Commission of the People′s Republic of China and the National Institute of Occupational Health and Poison Control, Chinese Center for Disease Control and Prevention. And the normative reference file related information was extracted to build standard coupling matrix. UCINET6 software for Windows was used to compute the node feature specifications. The network topology diagram of standard mutual citation relationship was drawn using Netdraw 2.054. RESULTS: A total of 269 national occupational health standards were included, involving 361 normative references files. Among them, the highest degree centrality was 48.064 in GBZ/T 251 Guidelines for Prevention and Control of Occupational Hazard in Foundry of Automobile Manufacturing. The highest closeness centrality and betweenness centrality were 27.386 and 33.210, respectively, in GBZ 2.1 Occupational Exposure Limits for Hazardous Agents in the Workplace--Part 1 : Chemical Hazardous Agents. The topographic map of standard mutual reference network indicated that five national occupational health standards were located in the central position of the mutual reference network, which included GBZ 1 Hygiene Standards for the Design of Industrial Enterprises, GBZ 2.1 Occupational Exposure Limits for Hazardous Agents in the Workplace--Part 1: Chemical Hazardous Agents, GBZ 2.2 Occupational Exposure Limits for Hazardous Agents in the Workplace--Part 2: Physical Agents, GBZ 158 Warning Sign for Occupational Hazards in the Workplace and GBZ 159 Specifications of Air Sampling for Hazardous Substances Monitoring in the Workplace. CONCLUSION: The five national occupational health standards located in the central position of the mutual reference network were the key nodes that have a greater influence on other standards, and can be the focus of future standard management.

Progress of classification algorithms for motor imagery electroencephalogram signals / 生物医学工程学杂志

Motor imagery (MI), motion intention of the specific body without actual movements, has attracted wide attention in fields as neuroscience. Classification algorithms for motor imagery electroencephalogram (MI-EEG) signals are able to distinguish different MI tasks based on the physiological information contained by the EEG signals, especially the features extracted from them. In recent years, there have been some new advances in classification algorithms for MI-EEG signals in terms of classifiers versus machine learning strategies. In terms of classifiers, traditional machine learning classifiers have been improved by some researchers, deep learning and Riemannian geometry classifiers have been widely applied as well. In terms of machine learning strategies, ensemble learning, adaptive learning, and transfer learning strategies have been utilized to improve classification accuracies or reach other targets. This paper reviewed the progress of classification algorithms for MI-EEG signals, summarized and evaluated the existing classifiers and machine learning strategies, to provide new ideas for developing classification algorithms with higher performance.

Analysis on human brucellosis from national reports in China, 2006-2016 / 中华劳动卫生职业病杂志

Objective@#To characterize the features of human brucellosis from national reports of occupational diseases and infectious diseases in China during 2006-2016, and explore the correlations between human brucellosis with the livestock and their products.@*Methods@#From January to October 2018, the data of human brucellosis were extracted from China's annual national reports of occupational diseases and infectious diseases from government circulars during 2006-2016. The data on the number of livestock and the output of livestock products were obtained from China Statistical Yearbook, China Animal Industry Yearbook and China Animal Industry and Veterinary Yearbook. The standards of Diagnosis of occupational infectious disease (GBZ 227-2017) and Diagnostic criteria for brucellosis (WS 269-2007) were compared. The statistics were analyzed using Excel 13.0 and SPSS software 22.0. The linear regression and multiple regression were used to explore the relationships between human brucellosis with the number of livestock and the output of livestock products.@*Results@#The diagnosis of human brucellosis in national reports of occupational diseases was based on GBZ 227-2017, which referred to WS 269-2007. There is an increasing trend from 2006 to 2009, and then declined to 2011, but rebounded from 2012 onwards in human brucellosis in national reports of occupational diseases. The general trend showed an increase during the 2006-2014 period, and then a decrease onwards in human brucellosis in national reports of infectious diseases. The Spearman correlation from SPSS showed the rs value of 0.936 (P<0.001) between human brucellosis cases in national reports of occupational diseases and infectious diseases in China from 2006 to 2016. Human brucellosis had a seasonal and periodic trend, and the cases revealed an epidemic period from April to August, with a peak in June. Human brucellosis cases were positively correlated with the number of cattle and buffaloes, hogs, sheep and goats (P<0.05) , and the output of pork, beef and mutton (P<0.05) . And human brucellosis cases in national reports of infectious diseases were positively associated with the output of sheep wool (β=0.814) , goat wool (β=0.603) , and cashmere (β=0.562) .@*Conclusion@#Human brucellosis both in national reports of occupational diseases and infectious diseases remain severe in China. There are relationships between human brucellosis with the number of livestock and the output of livestock products.

Initial exploration of discordance in public health standards in China / 中华流行病学杂志

Discordance, such as overlap, repetition and inconsistent, of standards is one of the major problems in current standardization affair in China. Therefore, improving the unity and authority of standards through reduction of overlap, repetition and inconsistency has become the main goal of deepening standardization reform in China. This paper summarizes the discordance in public health standards in China, analyzes the major reasons and provides specific strategic suggestions through case analysis of public health standards in the ways of comparisons of same kind standards of other deparments and standards in administration documents and guidelines or technical specifications of academic associations or societies.

The development trend of geriatric nursing and combination of medical and nursing by analysis of the keywords of literature in China / 中国实用护理杂志

Objective@#Through research on the geriatric nursing related literature in Chinese journals and medical care and elderly care combination related keywords from 1979 to 2017, this study provides the development trend of geriatric nursing research.@*Methods@#Searching articles in Wanfang, CNKI, VIP database, selecting the keywords and published time of the paper. Employing descriptive statistics to describe literatures information such as publication time, quantity of keywords, word frequency of keywords, heat degree of keywords, character of keywords and so on.@*Results@#Totally 134 791 articles were got and 58 031 articles were included in the analysis. The amount of literature growth was slow before 2005, The cumulative amount of literature in 1979-2005 was only 12.75% of the total amount of literature. After 2005, the amount of literature increased rapidly year by year. The number of literature published in 2017 has exceeded 12%. The amount of the geriatric nursing related literature was proportional to the number of people aged 65 and over in China. In the research of geriatric nursing, the content related to medical care and elderly care combination was quite dispersed, and it did not involve research at the level of Medical Association. The medical care and elderly care combination and Medical Association were new areas in the research of geriatric nursing.@*Conclusion@#The amount of the geriatric nursing related literature from 1979 to 2017 was proportional to the number of people aged 65 and over in China. There are few researches on how to carry out geriatric nursing under the policy of medical care and elderly care combination and Medical Association, which should be the focus of future research and exploration to actively respond to the challenge of aging population.

The development trend of geriatric nursing and combination of medical and nursing by analysis of the keywords of literature in China / 中国实用护理杂志

Objective Through research on the geriatric nursing related literature in Chinese journals and medical care and elderly care combination related keywords from 1979 to 2017, this study provides the development trend of geriatric nursing research. Methods Searching articles in Wanfang, CNKI, VIP database, selecting the keywords and published time of the paper. Employing descriptive statistics to describe literatures information such as publication time, quantity of keywords, word frequency of keywords, heat degree of keywords, character of keywords and so on. Results Totally 134 791 articles were got and 58 031 articles were included in the analysis. The amount of literature growth was slow before 2005,The cumulative amount of literature in 1979-2005 was only 12.75% of the total amount of literature. After 2005, the amount of literature increased rapidly year by year. The number of literature published in 2017 has exceeded 12% . The amount of the geriatric nursing related literature was proportional to the number of people aged 65 and over in China. In the research of geriatric nursing, the content related to medical care and elderly care combination was quite dispersed, and it did not involve research at the level of Medical Association. The medical care and elderly care combination and Medical Association were new areas in the research of geriatric nursing. Conclusion The amount of the geriatric nursing related literature from 1979 to 2017 was proportional to the number of people aged 65 and over in China. There are few researches on how to carry out geriatric nursing under the policy of medical care and elderly care combination and Medical Association, which should be the focus of future research and exploration to actively respond to the challenge of aging population.

A bibliometric analysis of China’s occupational health standard from 2001 to 2016 / 中华劳动卫生职业病杂志

Objective@#To analyze the relevant literature on China’s occupational health standard from 2001 to 2016, and to provide a basis for further improving China’s occupational health standard system.@*Methods@#Wanfang Data, CNKI, VIP, PubMed, Embase, and Web of Knowledge were used to retrieve relevant literature on China’s occupational health standard from 2001 to 2016. Information of the literature was collected, and type of literature, time and region, first author’s affiliation, and research funding were analyzed by bibliometrics.@*Results@#The relevant literature on China's occupational health standard was concentrated domestically and in the health system, and the number of documents was highly correlated with socio-economic conditions. The literature was dominated by the research on onsite application of occupational health (53.08%) , while there was less research on occupational health standard (11.78%) . The number of published articles and funding intensity were significantly different between centers for disease control and prevention at different levels.@*Conclusion@#It is suggested to accelerate the application of evidence-based medicine and systematic review in the development of occupational health standard, enhance the research ability of county/district-level disease control officers, assist them in summarizing and publishing their daily work experience, and give them appropriate funding, so as to promote the accumulation of knowledge in the field of occupational health.

Systematic analysis on legal system of workplace violence prevention and control in Chinese medical institutions / 中国职业医学

OBJECTIVE: To systematically analyze the related laws and regulations on workplace violence in Chinese medical institutions and provide recommendations for making policy. METHODS: The legal databases of CNKI,Wanfang,Lawyee and Pkulaw were retrieved by keywords including workplace violence,medical violence,medical dispute,doctor-patient dispute or medical order. The laws and regulations,department measures,local laws and government regulations were collected and systematically analyzed. RESULTS: In China,there are eight laws,two administrative regulations,two department rules and 15 provincial local laws and regulations stipulating that the healthcare workers enjoy the right to be free from workplace violence and constitute the legal basis for the prevention of medical violence in the workplace. During 2009-2017,the National Health and Family Planning Commission joints with multi-sector authorities have issued 12 notices related to the maintenance of medical order,from three aspects of human defense,physical defense and technical defense to vigorously emphasize the punishment of violent crime against personal safety of healthcare workers. However,compared to the international community,there is still a gap between China's legislation on the prevention and control of violence in medical workplaces,and there is no coherence in the definition of violence against the workplace. There are no specific legal and technical standards,and the occupational hazards caused by mental violence have not yet been included in the scope of legal adjustment and the national occupational disease classification directory. CONCLUSION: Specific legislation on prevention and treatment of medical institutions workplace violence should be carried out by using effective measures. The establishment of standard system,the related research and international exchange should be strengthened.

Relationship Between Serum DNA Replication, Clinicopathological Characteristics and Prognosis of Hepatitis B Virus-associated Glomerulonephritis with Severe Proteinuria by Lamivudine Plus Adefovir Dipivoxil Combination Therapy / 生物医学与环境科学（英文）

<p><b>OBJECTIVE</b>To explore the relationship between HBV DNA and the clinical manifestations, pathological types, injury severity, and prognosis with HBV-GN.</p><p><b>METHODS</b>102 patients with HBV-GN were divided into 3 groups, according to the serum titer of the HBV DNA. 24-h urine protein excretion, and other parameters were measured. Renal biopsy were performed. The association between HBV DNA and the pathological stage of membranous nephropathy was analyzed in 78 patients with HBV-MN. 24-h urine protein excretion was used for the evaluation of the prognosis, and the relationship between HBV DNA and prognosis were analyzed.</p><p><b>RESULTS</b>Several findings were demonstrated with the increase of serum HBV DNA: 24-h urine protein excretion, plasma cholesterol, and triglycerides increased significantly (P%lt;0.05), while the plasma level of albumin decreased significantly (P%lt;0.05); The changes of serum creatinine, C3 and C4 were found but no statistical significance. Glomerular deposition of HBVAg increased, and the pathological injury was more severe. The clinical remission rate was lower in the high replication group after treatment as compared with the low replication group (P%lt;0.01).</p><p><b>CONCLUSION</b>With the increase of serum HBV DNA, the urine protein excretion and the kidney injury were more severe, and the clinical remission rate was decreased.</p>

Correlation of serum HBV DNA level with clinicopathological characteristics and prognosis of hepatitis B virus-associated glomerulonephritis / 中华临床感染病杂志

Objective To investigate the correlation of HBV DNA level with clinicopathological characteristics and prognosis of HBV-associated glomerulonephritis ( HBV-GN ) .Methods One hundred and two patients with HBV-GN admitted in Affiliated Hospital of Qingdao University during January 2009 and October 2012 were successively enrolled.Fluorescence quantitative polymerase chain reaction was used to determine the serum titer of HBV DNA.According to HBV DNA levels the patients were divided into three groups:low-replication group (HBV DNA 105 copies/mL) .Renal biopsy was performed to determine the pathological type, and immunofluorescence assay was used for quantitative detection of HBV related antigens ( HBsAg, HBcAg and HBeAg ) in kidney. All patients received lamivudine (100 mg/d) plus adefovir dipivoxil (10 mg/d) combination therapy and followed up for 18 months.The renal function, biochemical and immunological indexes before and after the treatment were measured.One-way ANOVA was used to analyze the differences of above parameters among patients in three groups.Spearman correlation coefficient was used for correlation analysis between HBV DNA level and pathological stages in kidney.Results There were 20 patients in low-replication group, 51 in moderate-replication group and 31 in high-replication group.With the increase of serum level of HBV DNA, 24-h urine protein excretion, plasma cholesterol, and triglycerides increased (F=34.64, 40.10 and 31.72, P0.05);no change in creatinine was found (t =0.14, 0.52 and 0.57, P >0.05).After 18-month treatment, clinical remission rates in three groups were 95.0% ( 19/20 ) , 70.6% ( 36/51 ) and 54.8%(17/31), respectively.The clinical remission rate was significantly lower in the high-replication group as compared with that in low-replication group (χ2 =9.44, P<0.01).Conclusion Serum level of HBV DNA is closely correlated with renal function, renal pathology and clinical remission rate in HBV-GN patients, which may be used for the evaluation of kidney biopsy, treatment and prognosis in patients with HBV-GN.